BUB3.1 Antibody

Code CSB-PA126861XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BUB3.1 Polyclonal antibody
Uniprot No.
Target Names
BUB3.1
Alternative Names
BUB3.1 antibody; At3g19590 antibody; MMB12.5 antibody; Mitotic checkpoint protein BUB3.1 antibody; Protein BUDDING UNINHIBITED BY BENZYMIDAZOL 3.1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BUB3.1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. Essential for gametophyte development.
Gene References into Functions
  1. In Arabidosis, there is high similarity of Bub3.1 and Bub3.2 sequences at the nucleotide level but Bub3.1 and not Bub3.2 showed increased expression during mitosis. Bub3.1 appeared to be essential during embryogenesis. [Bub3.1] PMID: 18508582
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cytoskeleton, spindle. Note=Accumulates onto both kinetochores and the spindle microtubules in cell arrested in metaphase. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. Associates with unattached kinetochores upon spindle assembly checkpoint (SAC) activation. Present in the phragmoplast midline during the final step of cell division.
Protein Families
WD repeat BUB3 family
Tissue Specificity
Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems, flower buds, flowers and siliques.
Database Links

KEGG: ath:AT3G19590

STRING: 3702.AT3G19590.1

UniGene: At.8134

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