BUB3.3 Antibody

Code CSB-PA883275XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BUB3.3 Polyclonal antibody
Uniprot No.
Target Names
BUB3.3
Alternative Names
BUB3.3 antibody; At1g69400 antibody; F10D13.23 antibody; F23O10.3 antibody; Mitotic checkpoint protein BUB3.3 antibody; Protein BUDDING UNINHIBITED BY BENZYMIDAZOL 3.3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BUB3.3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20.
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cytoskeleton, spindle.
Protein Families
WD repeat BUB3 family
Database Links

KEGG: ath:AT1G69400

STRING: 3702.AT1G69400.1

UniGene: At.35426

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