Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BZIP11 Polyclonal antibody

Uniprot No.

O65683

Target Names

BZIP11

Alternative Names

BZIP11 antibody; ATB2 antibody; GBF6 antibody; At4g34590 antibody; T4L20.170bZIP transcription factor 11 antibody; AtbZIP11 antibody; G-box-binding factor 6 antibody; bZIP transcription factor ATB2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana BZIP11 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Transcription factor that binds to the DNA sequence 5'-ACTCAT-3' in target gene promoters. Promotes POX1/PRODH1 expression in response to hypoosmolarity stress. Positively regulates the expression of ASN1 and POX2/PRODH2 genes, which are involved in amino acid metabolism. Regulates several metabolic pathways such as myo-inositol, raffinose and trehalose. Regulates several trehalose metabolism genes, including TRE1, TPP5 and TPP6. Mediates recruitment of the histone acetylation machinery to activate auxin-induced transcription. Interacts with ADA2B adapter protein to promote ADA2B-mediated recruitment of SAGA-like histone acetyltransferase complexes to specific auxin-responsive genes.

Gene References into Functions

Data show that bZIP11 uORF2 encodes a regulatory nascent peptide that functions to sense intracellular sucrose abundance. PMID: 28369795
Based on ensuing molecular and biochemical analyses, we propose a mechanistic model, in which bZIP11-related TFs gain control over the root meristem by directly activating IAA3/SHY2 transcription. IAA3/SHY2 is a pivotal negative regulator of root growth, which has been demonstrated to efficiently repress transcription of major auxin transport facilitators of the PIN-FORMED (PIN) gene family, thereby restricting polar auxi PMID: 28158182
Arabidopsis bZIP11-related basic leucine zipper (bZIP) transcription factors interact via an amino-terminal activation domain with ADA2b adapter proteins to recruit the histone acetylation machinery to specific auxin-responsive genes. PMID: 24861440
The bZIP11 induction leads to reduced contents of the prominent growth regulatory molecule trehalose 6-phosphate (T6P). The metabolic changes detected mimic in part those observed in carbon-starved plants. PMID: 21534971
The uORF2 element in the bZip11 mRNA encodes a sucrose control peptide that inhibits bZIP11 translation in response to high sucrose levels by stalling the ribosome on the mRNA. PMID: 19403731

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Subcellular Location

Nucleus.

Tissue Specificity

Highly expressed in stems and flowers. Expressed in root tips, cotyledons, leaf vasculature, embryos, apical parts of siliques and funiculi.

Database Links

KEGG: ath:AT4G34590

STRING: 3702.AT4G34590.1

UniGene: At.135

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BZIP11 Antibody

Product Details

Customer Reviews and Q&A

Target Background