BZIP53 Antibody

Code CSB-PA968961XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BZIP53 Polyclonal antibody
Uniprot No.
Target Names
BZIP53
Alternative Names
BZIP53 antibody; At3g62420 antibody; T12C14.120bZIP transcription factor 53 antibody; AtbZIP53 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BZIP53 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator that binds DNA to the C-box-like motif (5'-TGCTGACGTCA-3'), ABRE elements, G-box-like motif (5'-CCACGTGGCC-3'), DOF (5'-AAAG-3'), I-box (5'-GATAA-3'), BS1 (5'-AGCGGG-3'), MY3 (5'-CGACG-3'), 5'-CAGTGCGC-3' and 5'-ACTCAT-3' sequence in target gene promoters. DNA-binding and subsequent transcription activation is triggered by heterodimerization with other bZIP proteins (e.g. BZIP1, BZIP10 and BZIP25). Promotes POX1/PRODH1 expression in response to hypoosmolarity stress. Transcriptional activator of seed maturation (MAT) genes (e.g. AT2S2), including seed storage protein (SSP) and late embryogenesis abundant (LEA) genes. Activated by low energy stress both by transcriptional and post-transcriptional mechanisms. Promotes dark-induced senescence and participates in the transcriptional reprogramming of amino acid metabolism during the dark-induced starvation response, especially when heterodimerized with BZIP1, by triggering accumulation of specific proteins including ASN1 and POX1/PRODH1.
Gene References into Functions
  1. Data show that the group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 reprogram primary C- and N-metabolism. PMID: 26276836
  2. bZIP1 and bZIP53 expression is enhanced during dark-induced starvation. Heterodimerization with members of the partially redundant C/S1 bZIP factor network reprograms primary metabolism in the starvation response. PMID: 21278122
  3. Heterodimers containing bZIP53 participate in enhanceosome formation to produce a dramatic increase in seed maturation gene transcription. PMID: 19531597
Subcellular Location
Nucleus.
Protein Families
BZIP family
Tissue Specificity
Expressed in developing seeds.
Database Links

KEGG: ath:AT3G62420

STRING: 3702.AT3G62420.1

UniGene: At.48798

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