CYP85A2 Antibody

Datasheet

Code	CSB-PA846372XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CYP85A2 Polyclonal antibody

Uniprot No.

Q940V4

Target Names

CYP85A2

Alternative Names

CYP85A2 antibody; BR6OX2 antibody; At3g30180 antibody; T20F20.9 antibody; T20F20_6Cytochrome P450 85A2 antibody; EC 1.14.-.- antibody; Brassinosteroid-6-oxidase 2 antibody; BR6ox 2 antibody; C6-oxidase 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CYP85A2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Catalyzes the C6-oxidation step in brassinosteroids biosynthesis. Converts 6-deoxocastasterone to castasterone, and castasterone to brassinolide. May also convert 6-deoxoteasterone to teasterone, 3-dehydro-6-deoxoteasterone to 3-dehydroteasterone, and 6-deoxotyphasterol to typhasterol.

Gene References into Functions

CYP85A2, the cytochrome P450 enzyme that performs the last step in brassinosteroid biosynthesis (conversion of castasterone to brassinolide)(4), must be farnesylated to function in Arabidopsis. Loss of either CYP85A2 or CYP85A2 farnesylation results in reduced brassinolide accumulation and increased plant responsiveness to the hormone abscisic acid (ABA) and overall drought tolerance, explaining previous observations. PMID: 27455172
The seu cyp85A2 double mutants displayed enhanced disruptions of gynoecial and ovule development. PMID: 20836864
investigated the temporal and spatial expression patterns of CYP85A1 and CYP85A2; both promoters of CYP85A1 and CYP85A2 showed maximum activity during the first week following germination, particularly in the vascular tissues PMID: 15821873
CYP85A2 is a bifunctional enzyme that possesses BR C-6 oxidase (C-6 oxidation of brassinosteroids) and brassinolide (BL) synthase activity. It is thus a cytochrome P450 that mediates Baeyer-Villiger oxidation in plants. [CYP85A2] PMID: 16024588
CYP85A2 catalyzes the lactonization reactions of not only castasterone but also teasterone and typhasterol PMID: 18685225

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Subcellular Location

Membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Tissue Specificity

Expressed in stems, leaves, siliques, shoots, and roots, with a higher expression in apical shoots.

Database Links

KEGG: ath:AT3G30180

STRING: 3702.AT3G30180.1

UniGene: At.26493

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CYP85A2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background