CYP94B3 Antibody

Datasheet

Code	CSB-PA227812XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CYP94B3 Polyclonal antibody

Uniprot No.

Q9SMP5

Target Names

CYP94B3

Alternative Names

CYP94B3 antibody; At3g48520 antibody; T8P19.30 antibody; Cytochrome P450 94B3 antibody; EC 1.14.14.48 antibody; Jasmonoyl-L-amino acid 12-hydroxylase antibody; Jasmonoyl-isoleucine-12-hydroxylase antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CYP94B3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

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Target Background

Function

Hydroxylase involved in the oxidation of the plant hormone jasmonoyl-L-isoleucine (JA-Ile), a bioactive phytohormone of the jasmonate-mediated signaling pathway. Converts JA-Ile to 12-hydroxy-JA-Ile. Exerts negative feedback control on JA-Ile levels and plays a key role in attenuation of jasmonate responses. Negatively regulates the expression of wound-induced genes TIFY11A/JAZ5, TIFY5A/JAZ8 and TIFY5A/JAZ10. Catalyzes the hydroxylation of jasmonoyl-L-valine (JA-Val), jasmonoyl-L-leucine (JA-Leu) and jasmonoyl-L-phenylalanine (JA-Phe) in vitro. Converts JA-Val, JA-Leu and JA-Phe to 12-hydroxy-JA-Val, 12-hydroxy-JA-Leu and 12-hydroxy-JA-Phe, respectively.

Gene References into Functions

It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds.(cytohrome P-450 94B3) PMID: 24467969
CYP94B3 plays a major regulatory role in controlling the level of jasmonyl-L-isoleucine in plants. PMID: 21849397

Subcellular Location

Membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Database Links

KEGG: ath:AT3G48520

STRING: 3702.AT3G48520.1

UniGene: At.43897

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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