CYP94C1 Antibody

Datasheet

Code	CSB-PA920565XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CYP94C1 Polyclonal antibody

Uniprot No.

Q9ZUX1

Target Names

CYP94C1

Alternative Names

CYP94C1 antibody; At2g27690 antibody; F15K20.21 antibody; Cytochrome P450 94C1 antibody; EC 1.14.14.49 antibody; 12-hydroxyjasmonoyl-L-amino acid 12-hydroxylase antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CYP94C1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Involved in the oxidation of the plant hormone jasmonoyl-L-isoleucine (JA-Ile), a bioactive phytohormone of the jasmonate-mediated signaling pathway. Converts 12-hydroxy-JA-Ile (12OH-JA-Ile) to the carboxy-derivative 12COOH-JA-Ile. Exerts negative feedback control on JA-Ile levels and plays a role in attenuation of jasmonate responses. Functions also as in-chain fatty acids hydroxylase in vitro. Catalyzes the hydroxylation of 12-hydroxy-jasmonoyl-L-phenylalanine (12OH-JA-Phe) in vitro. Converts 12OH-JA-Phe to the carboxy-derivative 12COOH-JA-Phe.

Gene References into Functions

we created the double and triple knock-out mutants of three CYP94 genes, CYP94B1, CYP94B3, and CYP94C1. The mutations blocked the oxidation steps and caused JA-Ile to accumulate 3-4-fold the WT levels in the wounded leaves. PMID: 26968098
CYP94B3 and CYP94C1 are responsible for the production of oxidized derivatives of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine in wounded plants. PMID: 26164240
These results highlight the dual function of CYP94B3/C1 in antimicrobial defence. PMID: 25903915
These results identify CYP94B3/C1-mediated oxidation as a major catabolic route for turning over the JA-Ile hormone. PMID: 22215670

Subcellular Location

Membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Database Links

KEGG: ath:AT2G27690

STRING: 3702.AT2G27690.1

UniGene: At.13273

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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