Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CALS12 Polyclonal antibody

Uniprot No.

Q9ZT82

Target Names

CALS12

Alternative Names

CALS12 antibody; GSL5 antibody; PMR4 antibody; At4g03550 antibody; F9H3.18 antibody; T5L23.4Callose synthase 12 antibody; EC 2.4.1.34 antibody; 1,3-beta-glucan synthase antibody; Protein GLUCAN SYNTHASE-LIKE 5 antibody; Protein POWDERY MILDEW RESISTANT 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CALS12 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in sporophytic and gametophytic development. Required for normal leaf development. During pollen formation, required for the formation of the callose wall separating the tetraspores of the tetrad (interstitial wall), but not for the callose wall surrounding the pollen mother cells (peripheral wall). Functionally redudant to CALS11 (GSL1). May play a role later in pollen grain maturation. Required for callose formation induced by wounding and pathogen attack. May interfere with salicylic acid-induced signaling pathway during defense response. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.

Gene References into Functions

RabA4c directly interacts with PMR4, which can be seen as an effector of this GTPase. PMID: 25056861
Potentiation of callose deposition and the heightened disease resistance phenotype in ocp3 plants required the plant hormone abscisic acid and the PMR4 gene encoding a callose synthase. PMID: 21564353
HrpN Ea-induced deterrent effect on phloem feeding of the green peach aphid Myzus persicae requires AtGSL5 and AtMYB44 genes in Arabidopsis thaliana. PMID: 21451254
EDR1 and GSL5 negatively regulate salicylic acid and jasmonate production or signaling by independent mechanisms and that negative regulation of defense signaling by GSL5 may be independent of callose production. PMID: 20367466

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Glycosyltransferase 48 family

Tissue Specificity

Highly expressed in flowers. Expressed at low levels in roots, leaves, stems, cauline leaves and siliques.

Database Links

KEGG: ath:AT4G03550

STRING: 3702.AT4G03550.1

UniGene: At.3956

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CALS12 Antibody

Product Details

Customer Reviews and Q&A

Target Background