CalpB Antibody

Code CSB-PA894270XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) CalpB Polyclonal antibody
Uniprot No.
Target Names
CalpB
Alternative Names
CalpB antibody; CG8107Calpain-B antibody; EC 3.4.22.- antibody; Calcium-activated neutral proteinase B antibody; CANP B) [Cleaved into: Calpain-B catalytic subunit 1; Calpain-B catalytic subunit 2] antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) CalpB protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Calcium-regulated non-lysosomal thiol-protease.
Gene References into Functions
  1. Autolytic activation, expression pattern and localization of calpain B. PMID: 14614768
  2. the acidic loop and the transducer region of calpain form an interconnected, extended structural unit that has the capacity to integrate and transduce Ca2+-evoked conformational changes over a long distance PMID: 14976200
  3. initial autolysis of Calpain B seems to occur intramolecularly, which supports the monomeric nature of Drosophila calpains PMID: 18032413
  4. CalpainB is required for AML1-ETO-induced blood cell disorders in Drosophila. PMID: 19581587
  5. the activation of the ERK pathway by extracellular signals results in the phosphorylation and activation of calpain B in fruit flies. PMID: 19694808

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Subcellular Location
Cytoplasm. Membrane. Note=Translocates to intracellular membranes when calcium levels are increased.
Protein Families
Peptidase C2 family
Tissue Specificity
Strongly expressed in follicular and border cells of the oocyte. Ubiquitously expressed in early embryos. Localized to the trachea and their orifices, and to the larynx of late embryos. Restricted to the salivary gland in third instar larvae.
Database Links

KEGG: dme:Dmel_CG8107

STRING: 7227.FBpp0076072

UniGene: Dm.2874

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