Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans unc-31 Polyclonal antibody

Uniprot No.

Q23658

Target Names

unc-31

Alternative Names

unc-31 antibody; ZK897.1Calcium-dependent secretion activator antibody; Uncoordinated protein 31 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans unc-31 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles. Specifically required to activate the neuronal G-alpha pathway. Functions with G-alpha proteins from the same motor neurons to regulate locomotion. Involved in regulating entry into quiescence triggered by satiety. Probably by regulating neuronal transmission downstream of lin-3 and receptor lin-23 and phospholipase plc-3 and upstream of innexin unc-7 and egl-4/PKG in ALA neurons, involved in the decrease in pharyngeal pumping during the quiescent state that precedes each larval molt. Plays a role in octopamine signaling and specifically, the octopamine inhibition of aversion responses in olfactory sensory neurons. Probably by controlling the secretion of FLP neuropeptides, regulates the turning step of male mating behavior.

Gene References into Functions

Four UNC-31 domains support distinct sequential molecular actions of UNC-31 in vesicular exocytosis, including steps in vesicle tethering and docking that bridge vesicle with plasma membrane. PMID: 20515653
UNC-31 is required for the docking of DCVs at the plasma membrane. Interestingly, the defect in DCV docking caused by UNC-31 mutation can be fully rescued by PKA activation. PMID: 18031683
CAPS function in dense core vesicle docking parallels UNC-13 in synaptic vesicle docking, which suggests that these related proteins act similarly to promote docking of independent vesicle populations. PMID: 18250196

Subcellular Location

Cytoplasmic vesicle membrane. Cell junction, synapse.

Tissue Specificity

Within the ventral, dorsal and sublateral nerve cords, it is concentrated in cholinergic synapses. Within the ventral and dorsal nerve cords, it is not concentrated at most non-cholinergic synapses in the nerve cords, such as the GABAergic, glutamatergic,

Database Links

STRING: 6239.ZK897.1c

UniGene: Cel.12250

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unc-31 Antibody

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