D20 Antibody

Code CSB-PA791821XA01FQT
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Product Details

Full Product Name
Rabbit anti-Escherichia phage T5 (Enterobacteria phage T5) D20 Polyclonal antibody
Uniprot No.
Target Names
D20
Alternative Names
D20 antibody; ORF138 antibody; T5.149 antibody; T5p145Major capsid protein antibody; Capsid protein pb8 antibody; Major head protein) [Cleaved into: Scaffolding domain delta] antibody
Raised in
Rabbit
Species Reactivity
Escherichia phage T5 (Enterobacteria phage T5)
Immunogen
Recombinant Escherichia phage T5 (Enterobacteria phage T5) D20 protein
Immunogen Species
Escherichia phage T5 (Enterobacteria phage T5)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Major capsid protein that self-associates to form 120 hexamers and 11 pentamers, building the T=13 icosahedral capsid which about 860 Angstroms in diameter. Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function. The capsid gains its final stability through the reorganization of the subunits that takes place upon expansion. DNA encapsidation through the portal triggers capsid expansion and the binding of the decoration protein to the capsid exterior.
Gene References into Functions
  1. These data indicate that pb8 carries all of the information in its primary sequence to assemble in vitro without the requirement of the portal and accessory proteins. PMID: 20573812
Subcellular Location
Virion.
Protein Families
HK97 phage major capsid protein family
Database Links

KEGG: vg:2777673

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