gp23 Antibody

Code CSB-PA361291XA01EDZ
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Product Details

Full Product Name
Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) gp23 Polyclonal antibody
Uniprot No.
Target Names
gp23
Alternative Names
gp23Major capsid protein antibody; Gene product 23 antibody; Major head protein antibody; gp23) [Cleaved into: Mature major capsid protein antibody; gp23*)] antibody
Raised in
Rabbit
Species Reactivity
Enterobacteria phage T4 (Bacteriophage T4)
Immunogen
Recombinant Enterobacteria phage T4 (Bacteriophage T4) gp23 protein
Immunogen Species
Enterobacteria phage T4 (Bacteriophage T4)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Major capsid protein that self-associates to form hexamers, building most of the capsid in association with pentons made of the capsid vertex protein and one dodecamer of the portal protein. The major capsid protein self-associates to form 160 hexamers, building most of the T=13 laevo capsid. Folding of major capsid protein requires the assistance of two chaperones, the host chaperone groL acting with the phage encoded gp23-specific chaperone, gp31. The capsid also contains two nonessential outer capsid proteins, Hoc and Soc, which decorate the capsid surface. Through binding to adjacent gp23 subunits, Soc reinforces the capsid structure.
Gene References into Functions
  1. The distribution of g23 of T4-type bacteriophages was distinctly different among natural environments of marines, lakes and paddy fields. PMID: 21866696
  2. N terminus of the head protein of T4 bacteriophage directs proteins to the GroEL chaperonin PMID: 15571729
  3. time-resolved fluorescence properties of the bacteriophage T4 capsid protein gp23 are investigated PMID: 15629249
  4. folding of the T4 major capsid protein gp23 requires a gp31-dependent cis-folding mechanism likely inside an enlarged "Anfinsen cage" provided by GroEL and gp31 PMID: 15919824
  5. structures of gp23-chaperonin complexes, showing both the initial captured state and the final, close-to-native state with gp23 encapsulated in the folding chamber PMID: 19122642

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Subcellular Location
[Major capsid protein]: Virion.; [Mature major capsid protein]: Virion.
Protein Families
Tevenvirinae major capsid protein family
Database Links

KEGG: vg:1258751

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