cpb Antibody

Code CSB-PA004514XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) cpb Polyclonal antibody
Uniprot No.
Target Names
cpb
Alternative Names
cpb antibody; ANCP-BETA antibody; CG17158F-actin-capping protein subunit beta antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) cpb protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Gene References into Functions
  1. Data show that actin-capping protein subunits Cpa and Cpb stabilize each other's protein levels and can stimulate the production of each other's mRNA when the level of one of the subunit is reduced. PMID: 24788460
  2. Data show that the actin-Capping Protein (CP) alphabeta heterodimer, which regulates actin filament (F-actin) polymerization, limits Src-induced apoptosis or tissue overgrowth by restricting JNK activation. PMID: 23644660
  3. Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis PMID: 23339103
  4. the actin-capping protein heterodimer, which regulates actin filament polymerization, has a dual function on DE-cadherin in restricted epithelia PMID: 21963538
  5. findings show an interdependency between Hpo signaling activity and F-actin dynamics in which CP and Hpo pathway activities inhibit F-actin accumulation, and the reduction in F-actin in turn sustains Hpo pathway activity PMID: 21525075
  6. Mutations in the Drosophila orthologs of the F-actin capping protein alpha- and beta-subunits cause actin accumulation and subsequent retinal degeneration. PMID: 16143599

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
F-actin-capping protein beta subunit family
Database Links

KEGG: dme:Dmel_CG17158

STRING: 7227.FBpp0077549

UniGene: Dm.4772

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