Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CATHB3 Polyclonal antibody

Uniprot No.

Q94K85

Target Names

CATHB3

Alternative Names

CATHB3 antibody; At4g01610 antibody; T15B16.17aCathepsin B-like protease 3 antibody; EC 3.4.22.- antibody; Cathepsin B3 antibody; AtCathB3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CATHB3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Thiol protease that possesses high activity toward the cathepsin synthetic substrate Arg-Arg-7-amino-4-methylcoumarin (RR-AMC) and the papain substrate Gly-Arg-Arg-AMC (GRR-AMC). Can cleave the papain substrate Phe-Arg-AMC (FR-AMC) and the caspase-3 substrate Asp-Glu-Val-Asp-rhodamine 110 (DEVD-R110). Has no activity towards the caspase-6 substrate VEID-AMC, caspase-8 substrate IETD-AMC and caspase-1 substrate YVAD-AMC. Plays a central role in plant programmed cell death (PCD). In addition to its role in protein degradation, may cleave and/or degrade a number of target proteins, activating signaling towards PCD. Contributes to the increase of caspase-3-like activity after UV-C-induced PCD and is required for abiotic stress-induced PCD. Functions redundantly with CATHB1 and CATHB2 in basal defense and distinct forms of plant programmed cell death (PCD). Participates in the establishment of basal resistance against the bacterial pathogen Pseudomonase syringae pv. tomato DC3000. Required for full levels of PCD during resistance (R) gene-mediated hypersensitive response (HR). Involved in the regulation of senescence, a developmental form of PCD in plants. May be involved in the degradation of seed storage proteins during seed germination.

Gene References into Functions

Recombinant and native AtCathB3 had enzymatic activity against the synthetic caspase-3 substrate DEVD (Asp-Glu-Val-Asp) and were inhibited by synthetic caspase-3 inhibitors. cathepsin B does regulate PCD in both animal and plant cells suggesting that this protease may be part of an ancestral PCD pathway pre-existing the plant/animal divergence. [Cathepsin B3] PMID: 27058316
GBF1 is a transcriptional repressor of the AtCathB3 gene and affects germination kinetics of A. thaliana seeds. As AtCathB3 is also expressed during post-germination in cotyledons, a role for the AtCathB3-like protease in reserve mobilization is inferred. PMID: 24600022

Subcellular Location

Vacuole.

Protein Families

Peptidase C1 family

Tissue Specificity

Expressed in root tips, root vasculature, emerging lateral root, hydathodes, vascular tissue of leaves, vasculature of sepals and anthers, stigma, and vascular bundles at the base and the upper part of the siliques.

Database Links

KEGG: ath:AT4G01610

STRING: 3702.AT4G01610.1

UniGene: At.24471

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CATHB3 Antibody

Product Details

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Target Background