cbpA Antibody

Code CSB-PA336200XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cbpA Polyclonal antibody
Uniprot No.
Target Names
cbpA
Alternative Names
cbpA antibody; b1000 antibody; JW0985 antibody; Curved DNA-binding protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cbpA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adaptive response to environmental stresses other than heat shock. Lacks autonomous chaperone activity; binds native substrates and targets them for recognition by DnaK. Its activity is inhibited by the binding of CbpM.
Gene References into Functions
  1. CbpA recognizes the minor groove of the double helix which is mediated by amino acid side chain R116, which sits at the boundary of the linker and C-terminal domain I. PMID: 25670677
  2. our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure PMID: 23341772
  3. Derivatives of CbpA that are unable to dimerize are also unable to bind DNA. PMID: 20633229
  4. These data demonstrate that CbpA activity is controlled at multiple levels. PMID: 18502857
Subcellular Location
Cytoplasm, nucleoid.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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