cbtA Antibody

Code CSB-PA354321XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cbtA Polyclonal antibody
Uniprot No.
Target Names
cbtA
Alternative Names
cbtA antibody; yeeV antibody; b2005 antibody; JW1987Cytoskeleton-binding toxin CbtA antibody; Toxin CbtA antibody; Toxin YeeV antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cbtA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Toxic component of a type IV toxin-antitoxin (TA) system. Acts as a dual toxin inhibitor that blocks cell division and cell elongation in genetically separable interactions with FtsZ and MreB. Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization and GTPase activity as well as MreB ATP-dependent polymerization. Binds to both the N- and C-terminus of FtsZ, likely blocking its polymerization and localization, leading to blockage of cell division. Overexpression results in inhibition of growth in liquid cultures and decrease in colony formation; these effects are overcome by concomitant expression of antitoxin CbeA (YeeU). In other experiments cells swell, by 6 hours are lemon-shaped and by 24 hours those that have not lysed are spherical with diminished polar regions. Toxic effects are neutralized by cognate antitoxin CbeA, although there is no direct interaction between the 2 proteins. Toxic effects are also neutralized by overexpression of noncogate antitoxins YafW and YpjF.
Subcellular Location
Cytoplasm.
Protein Families
CbtA/YkfI/YpjF toxin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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