CCD1 Antibody

Code CSB-PA527730XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CCD1 Polyclonal antibody
Uniprot No.
Target Names
CCD1
Alternative Names
CCD1 antibody; NC1 antibody; NCED1 antibody; At3g63520 antibody; MAA21_150Carotenoid 9,10(9',10')-cleavage dioxygenase 1 antibody; EC 1.14.99.n4 antibody; AtCCD1 antibody; Neoxanthin cleavage enzyme NC1 antibody; AtNCED1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CCD1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cleaves a variety of carotenoids symmetrically at both the 9-10 and 9'-10' double bonds. Active on beta,beta-carotene, lutein, zeaxanthin, all-trans-violaxanthin, 9-cis-violaxanthin and 9'-cis-neoxanthin. With most substrates, the carotenoid is symmetrically cleaved. Probably not involved in abscisic acid biosynthesis.
Gene References into Functions
  1. AtCCD1 from Arabidopsis thaliana is a dioxygenase PMID: 16459333
  2. Ectopic expression of AhNCED1 gene in 129B08/nced3 mutant Arabidopsis driven by the AtNCED3 promoter restores its ability to accumulate ABA during drought stress, and reverts its hypersensitivity to nonionic osmotic stress and soil drought. PMID: 16870153
  3. A cysteine residue may modulate substrate affinity and (or) the reaction rate of AtCCD1. PMID: 18523487
Subcellular Location
Cytoplasm. Note=On the exterior surface of the plastids.
Protein Families
Carotenoid oxygenase family
Tissue Specificity
High expression in flowers and siliques. Also detected in stems, leaves and roots.
Database Links

KEGG: ath:AT3G63520

STRING: 3702.AT3G63520.1

UniGene: At.22615

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