Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CCD7 Polyclonal antibody

Uniprot No.

Q7XJM2

Target Names

CCD7

Alternative Names

CCD7 antibody; MAX3 antibody; NCED7 antibody; At2g44990 antibody; T14P1.21Carotenoid cleavage dioxygenase 7 antibody; chloroplastic antibody; AtCCD7 antibody; AtNCED7 antibody; Beta,beta-carotene 9',10'-oxygenase antibody; EC 1.13.11.68 antibody; Protein MORE AXILLARY BRANCHING 3 antibody; Protein MORE AXILLARY GROWTH 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CCD7 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in strigolactones biosynthesis by cleaving asymmetrically a variety of linear and cyclic carotenoids at the 9-10 double bond. Produces one C(13) beta-ionone and the C(27) 10'-apo-beta-carotenal. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. No activity on lycopene, lutein, zeaxanthin, violaxanthin or neoxanthin. Probably not involved in abscisic acid biosynthesis.

Gene References into Functions

enzymatic potential to form acyclic regulatory metabolites PMID: 27811075
The strigolactone biosynthesis genes MAX3 and MAX4 were drastically induced during dark incubation and treatment with the senescence-promoting phytohormone ethylene, suggesting that strigolactone is synthesized in the leaf during leaf senescence. PMID: 25979917
Substrate and stereo-specificity of the Arabidopsis CCD7 enzyme. PMID: 24685691
Wild-type and strigolactone mutants of A. thaliana Atccd7 and Atccd8 induced similar levels of P. ramosa seed germination, suggesting that compounds other than strigolactone function as germination stimulants for P. ramosa in other Brassicaceae spp. PMID: 22414435
The promoter activity of the 1,867-bp 5'-upstream region of AtCCD7exhibited a vascular specificity at all developmental stages throughout the transgenic Arabidopsis plants tested. PMID: 21243360
Results show that MAX3 and MAX4 genes were down-regulated in igi1 (Inflorescence Growth Inhibitor 1) mutants. PMID: 20473553
the sequential cleavages of beta-carotene by AtCCD7 and AtCCD8 may be initial steps in the synthesis of a carotenoid-derived signaling molecule necessary for the regulation lateral branching. [AtCCD7] PMID: 15342640
Complementation analyses for HTD1 confirm that the defect in HTD1 is responsible for both high-tillering and dwarf phenotypes in the htd1 mutant; HTD1 is an ortholog of MAX3 in A. thaliana. PMID: 17092317

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Subcellular Location

Plastid, chloroplast.

Protein Families

Carotenoid oxygenase family

Tissue Specificity

Expressed in flowers, siliques, inflorescence stems, petiole, leaves and roots.

Database Links

KEGG: ath:AT2G44990

STRING: 3702.AT2G44990.1

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CCD7 Antibody

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