ccmM Antibody

Code CSB-PA113882XA01FPY
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Product Details

Full Product Name
Rabbit anti-Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) ccmM Polyclonal antibody
Uniprot No.
Target Names
ccmM
Alternative Names
ccmM antibody; Synpcc7942_1423 antibody; Carbon dioxide concentrating mechanism protein CcmM antibody
Raised in
Rabbit
Species Reactivity
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Immunogen
Recombinant Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) ccmM protein
Immunogen Species
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions as a scaffold protein for the assembly of beta-carboxysomes, initiates carboxysome assembly by coalescing RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS). Produced as a full-length (M58) and a shorter form (M35); both forms are required for correct carboxysome assembly and growth. The short form is more abundant. Despite its strong similarity to gamma-class carbonic anhydrase (CA) it does not have detectable CA activity (Ref.3).; The M35 isoform is able to condense RuBisCO into a liquid matrix; the presence of disulfide bonds in M35 reduces affinity for RuBisCO, while mutating all 4 Cys to Ser causes a 4-fold increase in doubling time, more than 15% increase in CO(2) requirement, and abnormal carboxysomes.; Beta-carboxysome assembly initiates when soluble RuBisCO is condensed into a liquid matrix in a pre-carboxysome by the RbcS-like domains of probably both CcmM58 and CcmM35. CcmN interacts with the N-terminus of CcmM58, and then recruits the CcmK2 major shell protein plus other less abundant CcmK proteins via CcmN's encapsulation peptide. Shell formation requires CcmK proteins and CcmO. CcmL caps the otherwise elongated carboxysome. Once fully encapsulated carboxysomes are formed, they migrate within the cell probably via interactions with the cytoskeleton.
Subcellular Location
Carboxysome.
Protein Families
Gamma-class carbonic anhydrase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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