Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans cye-1 Polyclonal antibody

Uniprot No.

O01501

Target Names

cye-1

Alternative Names

cye-1 antibody; C37A2.4G1/S-specific cyclin-E antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans cye-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Essential for the control of the cell cycle at the G1/S (start) transition. In association with cdk-2, regulates proliferation, quiescent state and cell fate during the development of several cell lineages. In the embryo, initiates the establishment of cell polarity through the recruitment of the centrosomal proteins spd-2 and spd-5 during prophase. During the development of the vulva, controls the onset of vulval cell terminal differentiation by controlling the duration of G1 phase. During hypoderm development at early larval stages, controls syncytial fate of seam cell daughter cells. Involved in the progression of cell division in the intestinal lineage in larvae, and in particular in endoreplication, a specific growth pathway in the intestinal epithelium, required for feeding and gut development in growing larvae. By controlling the activity of translational repressor gld-1, regulates the pool of germline stem cells and the size of the mitotic zone by preventing entry into meiosis. In addition, repression of expression by gld-1 prevents mitosis re-entry in meiotic germline cells.

Gene References into Functions

results suggest that the balance between the levels of CKI and cyclin E determines three distinct cell states: terminally differentiated, quiescent and uncommitted, and proliferating PMID: 17476329
Notch and Prospero repress proliferation following cyclin E overexpression in bristle cell lineage. PMID: 19662164
GLD-1 represses translation of CYE-1/cyclin E during meiotic prophase, which prevents germ cells from re-entering mitosis and inducing embryonic-like transcription PMID: 19758560

Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.

Protein Families

Cyclin family, Cyclin E subfamily

Tissue Specificity

Expressed dynamically in proliferating cells throughout development. Detectable in larval blast cells undergoing active proliferation that give rise to all tissue types, including germline, intestine, hypodermis, neurons, and muscle.

Database Links

KEGG: cel:CELE_C37A2.4

STRING: 6239.C37A2.4a

UniGene: Cel.9296

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cye-1 Antibody

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