CCZ1 Antibody

Code CSB-PA330851XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CCZ1 Polyclonal antibody
Uniprot No.
Target Names
CCZ1
Alternative Names
CCZ1 antibody; AUT11 antibody; CVT16 antibody; YBR131W antibody; YBR1006Vacuolar fusion protein CCZ1 antibody; Autophagy-related protein 11 antibody; Calcium-caffeine-zinc sensitivity protein 1 antibody; Cytoplasm to vacuole targeting protein 16 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CCZ1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
In complex with MON1, is required for multiple vacuole delivery pathways including the cytoplasm to vacuole transport (Cvt), autophagy, pexophagy and endocytosis. The CCZ1-MON1 complex acts at the fusion of vesicles with the vacuole, through its regulation of the SNARE complex during the coordinated priming and docking stages of fusion, and particularly at the stage of tethering/docking. The CCZ1-MON1 complex acts as a guanine nucleotide-exchange factor (GEF) for Rab-type GTPase YPT7, promoting nucleotide exchange on YPT7 and triggering endosomal maturation by activating YPT7 on late endosomes.
Gene References into Functions
  1. Guanine nucleotide exchange factor activity of the Mon1-Ccz1 complex towards Ypt7 requires the interface formed by their longin domains. PMID: 24413168
  2. Data indicate that the Mon1-Ccz1 complex triggers endosomal maturation by activating Ypt7 on late endosomes. PMID: 20797862
  3. vacuolar sorting protein Ccz1p has multiple functions in Saccharomyces cerevisiae PMID: 15721293
  4. These results suggest that the Ccz1p-Mon1p-Ypt7p complex is involved in fusion of transport vesicles to multiple target membranes in yeast cells. PMID: 19456873
Subcellular Location
Endosome, multivesicular body membrane; Peripheral membrane protein. Prevacuolar compartment membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Note=The association of the CCZ1-MON1 complex with the vacuole is regulated by the C-Vps/HOPS complex.
Protein Families
CCZ1 family
Database Links

KEGG: sce:YBR131W

STRING: 4932.YBR131W

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