CDC27B Antibody

Code CSB-PA818596XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CDC27B Polyclonal antibody
Uniprot No.
Target Names
CDC27B
Alternative Names
CDC27B antibody; HBT antibody; At2g20000 antibody; T2G17.20 antibody; Cell division cycle protein 27 homolog B antibody; CDC27 homolog B antibody; Protein HOBBIT antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CDC27B protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication. Functionally redundant with CDC27A in the control of gametophyte development.
Gene References into Functions
  1. Small clones reveal non-cell-autonomous rescue of hbt mutant cells, indicating a previously unrecognized compensation mechanism for reduced activity of an anaphase-promoting complex/cyclosome component critical for cell cycle progression. PMID: 16938844
Subcellular Location
Nucleus.
Protein Families
APC3/CDC27 family
Tissue Specificity
Specifically expressed in dividing and elongating cells.
Database Links

KEGG: ath:AT2G20000

STRING: 3702.AT2G20000.1

UniGene: At.39758

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