cdc12 Antibody

Code CSB-PA605929XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) cdc12 Polyclonal antibody
Uniprot No.
Target Names
cdc12
Alternative Names
cdc12 antibody; SPAC1F5.04cCell division control protein 12 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) cdc12 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Plays a role in the cell cycle. Involved in cytokinesis. Component of the cell division ring. In the absence of profilin, caps the barbed end of actin filaments, thus preventing subunit addition and dissociation. In the presence of profilin, nucleates actin filaments that grow rapidly from their barbed ends.
Gene References into Functions
  1. Cdc12 mechanoregulation facilitates actomyosin ring assembly. PMID: 28951543
  2. this paper defines a short motif within the N terminus of Cdc12 that binds directly to the F-BAR domain of the scaffolding protein Cdc15. PMID: 25688133
  3. Cdc12 is an efficient nucleator (one filament per approximately 3 Cdc12 dimers) that processively elongates profilin-actin at a moderate rate of 10 subunits s-1 muM-1, but lacks filament-bundling activity. PMID: 21865598
  4. cdc12p contributes at least two separable activities: actin assembly and one or more additional functions in cytokinesis initiation PMID: 20226666
  5. A mutational analysis of cdc12p was used to identify regions of cdc12p responsible for ring assembly and localization. PMID: 18305104
  6. most likely hypotheses to explain the incompatibility of human profilin-I with Cdc12p are differences in interactions with the proline-rich sequences in the FH1 domain of Cdc12p and wider "wings" that interact with actin PMID: 19028693
  7. Cdc12p can recruit SpTm to actin filaments and SpTm has diverse effects on Cdc12p-mediated actin assembly. PMID: 19244341

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Subcellular Location
Nucleus. Note=Nuclear, and septum.
Protein Families
Formin homology family, BNI1 subfamily
Database Links
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