CDC73 Antibody

Code CSB-PA573724XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CDC73 Polyclonal antibody
Uniprot No.
Target Names
CDC73
Alternative Names
CDC73 antibody; PHP antibody; At3g22590 antibody; F16J14.15Protein CDC73 homolog antibody; Protein PLANT HOMOLOGOUS TO PARAFIBROMIN antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CDC73 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the PAF1 complex (PAF1C) which is involved in histone modifications such as methylation on histone H3 'Lys-4' (H3K4me3). Involved in regulation of flowering time. Required for the expression of the flowering repressors FLC and MADS-box genes of the MAF family. Required for histone H3 trimethylation on 'Lys-4' (H3K4me3) at the FLC locus. Prevents trimethylation on 'Lys-27' (H3K27me3) at the same locus.
Gene References into Functions
  1. PHP is an accessory subunit of Paf1C that promotes proper regulation of genes within H3K27me3-enriched chromatin. PMID: 21720211
  2. CDC73 is required for high levels of FLC expression in a subset of autonomous-pathway-mutant backgrounds and functions both to promote activating histone modifications (H3K4me3) as well as preventing repressive ones (e.g. H3K27me3). [CDC73] PMID: 20463090
  3. PLANT HOMOLOGOUS TO PARAFIBROMIN represents the plant homolog of parafibromin, and interacts physically with other Paf1C components. PMID: 20363855
Subcellular Location
Nucleus.
Protein Families
CDC73 family
Tissue Specificity
Expressed in root tips, shoot apex, young leaves and flowers, especially in stamen filaments and carpels.
Database Links

KEGG: ath:AT3G22590

STRING: 3702.AT3G22590.1

UniGene: At.28485

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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