cdiA4 Antibody

Code CSB-PA785179XA01FSJ
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Product Details

Full Product Name
Rabbit anti-Escherichia coli O157:H7 (strain EC869) cdiA4 Polyclonal antibody
Uniprot No.
Target Names
cdiA4
Alternative Names
cdiA4 antibody; cdiA-CTo11 antibody; ECH7EC869_5883Deoxyribonuclease CdiA-o11 antibody; DNase CdiA antibody; EC 3.1.-.- antibody; CdiA-o11-EC869 antibody; Toxin CdiA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli O157:H7 (strain EC869)
Immunogen
Recombinant Escherichia coli O157:H7 (strain EC869) cdiA4 protein
Immunogen Species
Escherichia coli O157:H7 (strain EC869)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. The C-terminal 289 residues (the CT fragment) has a strong DNase activity in the presence of Zn(2+), completely degrading supercoiled and linear plasmids, and inhibits growth. In the presence of Mg(2+) it nicks dsDNA. Toxic activity is neutralized by coexpression of the cognate immunity protein CdiI-o11-EC869, but not by non-cognate immunity proteins from other toxin-immunity modules or other strains of E.coli. Gains access to the cytoplasm of target cells by using integral inner membrane protein YciB.; Expression of this locus confers protection against other bacteria carrying the locus.
Subcellular Location
Target cell, target cell cytoplasm.
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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