cdk-2 Antibody

Code CSB-PA270612XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans cdk-2 Polyclonal antibody
Uniprot No.
Target Names
cdk-2
Alternative Names
cdk-2 antibody; K03E5.3Cyclin-dependent kinase 2 antibody; EC 2.7.11.22 antibody; Cell division protein kinase 2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans cdk-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase which, in association with cye-1, regulates proliferation, quiescent state and cell fate during the development of several cell lineages. In the embryo, initiates the establishment of cell polarity through the recruitment of the centrosomal proteins spd-2 and spd-5 during prophase. Phosphorylation and inhibition of the translational repressor gld-1 by the cdk-2/cye-1 complex regulates the pool of germline stem cells and the size of the mitotic zone in the gonads by preventing entry into meiosis.
Gene References into Functions
  1. CYE-1/CDK-2 emerges as a critical regulator of stem cell maintenance. We suggest that cyclin E and Cdk-2 may be used broadly to control developmental regulators. PMID: 21455289
  2. Rapid movement through the cell cycle is supported by constitutive CYE-1/CDK-2 activity; CYE-1 and CDK-2 promote the proliferative fate as RNAi depletion of cye-1 or cdk-2 in a glp-1 partial loss-of-function mutant causes premature meiotic entry. PMID: 21558371
  3. in wild-type animals, CKI-1/CKI in these cells maintained quiescence by inhibiting CYE-1/CDK-2, while sufficient CYE-1/CDK-2 remained to repress the terminal differentiation PMID: 17476329
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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