CPK5 Antibody

Code CSB-PA655034XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPK5 Polyclonal antibody
Uniprot No.
Target Names
CPK5
Alternative Names
CPK5 antibody; At4g35310 antibody; F23E12.130Calcium-dependent protein kinase 5 antibody; EC 2.7.11.1 antibody; Calmodulin-domain protein kinase CDPK isoform 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CPK5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
May play a role in signal transduction pathways that involve calcium as a second messenger.
Gene References into Functions
  1. The work uncovers a direct functional link between an atypical immune receptor and a crucial component of early immune signaling: increased immunity in exo70B1 depends on TN2 and CPK5 and, in a positive feedback loop, TN2 keeps CPK5 enzymatically active beyond the initiating stimulus. PMID: 28351987
  2. Results indicate that AtCPK5 is myristoylated at its amino terminus and that myristoylation is required for membrane binding. PMID: 23609608
  3. a model of ROS-mediated cell-to-cell communication, where a self-propagating mutual activation circuit consisting of the protein kinase, CPK5, and the NADPH oxidase RBOHD facilitates rapid signal propagation PMID: 23650383
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily
Database Links

KEGG: ath:AT4G35310

STRING: 3702.AT4G35310.1

UniGene: At.21676

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