CPK6 Antibody

Code CSB-PA655730XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CPK6 Polyclonal antibody
Uniprot No.
Target Names
CPK6
Alternative Names
CPK6 antibody; CDPK3 antibody; At2g17290 antibody; F5J6.13Calcium-dependent protein kinase 6 antibody; EC 2.7.11.1 antibody; Calcium-dependent protein kinase isoform CDPK3 antibody; AtCDPK3 antibody; Calmodulin-domain protein kinase CDPK isoform 6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CPK6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
May play a role in signal transduction pathways that involve calcium as a second messenger. Functions in abscisic acid (ABA) regulation of guard cell S-type anion- and Ca(2+)-permeable channels and stomatal closure. Phosphorylates FD.
Gene References into Functions
  1. (CPK6 and CPK33) are expressed in shoot apical meristem and directly interact with FD, suggesting they have redundant functions PMID: 25661797
  2. CPK6 positively functions in induction by yeast elicitor (YEL) of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis. PMID: 23922271
  3. These results provide genetic evidence that CPK6 has a different role from CPK3 and functions as a positive regulator of methyl jasmonate signaling in Arabidopsis guard cells. PMID: 20978156
  4. AtCPK6 is functionally redundant and a positive regulator involved in the tolerance to salt/drought stress in Arabidopsis. PMID: 20217124
  5. CPK6 functions in guard cell ion channel regulation and provide genetic evidence for calcium sensors that transduce stomatal ABA signaling. PMID: 17032064

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Subcellular Location
Cell membrane; Lipid-anchor. Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CDPK subfamily
Tissue Specificity
Expressed in both guard cells and mesophyll cells. Expressed in the shoot apical meristem.
Database Links

KEGG: ath:AT2G17290

STRING: 3702.AT2G17290.1

UniGene: At.9828

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