Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) cds1 Polyclonal antibody

Uniprot No.

Q09170

Target Names

cds1

Alternative Names

cds1 antibody; SPCC18B5.11cSerine/threonine-protein kinase cds1 antibody; EC 2.7.11.1 antibody; Checkpoint kinase cds1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) cds1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Has a role in the DNA replication-monitoring S/G2 checkpoint system. It is responsible for blocking mitosis in the S phase. It monitors DNA synthesis by interacting with DNA polymerase alpha and sends a signal to block the onset of mitosis while DNA synthesis is in progress. Phosphorylates rad60 and dna2.

Gene References into Functions

The prolongation in mutants deficient in meiotic DNA replication, was caused by the Cds1-dependent replication checkpoint, which represses expression of the mei4(+) gene encoding a meiosis-specific transcription factor. PMID: 25492408
Cki3 acts downstream of the checkpoint kinase Cds1/Chk2 and calcineurin, and the latter physically interacts with Cki3. PMID: 25691662
Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6. PMID: 19755492
the existence in fission yeast of a short variant (Sv) of Cds1 that is produced through an alternative splicing mechanism leading to a frame shift and premature termination PMID: 15467464
Replication checkpoint kinase Cds1 regulates Mus81 (a subunit of the Mus81-Eme1 structure-specific endonuclease) by phosphorylation, to preserve genome integrity during replication stress. PMID: 15805465
Rad3-Cds1 meiotic checkpoint pathway may target transcription of these factors. PMID: 16286472
DNA-damage checkpoint pathways mediated by Cds1 and Rad3. PMID: 18154680
These data show that by releasing Flp1p into the nucleus Cds1p checkpoint kinase modulates its own full activation during replication stress. PMID: 18385517
Clr6-HDAC (histone deacetylase) regulates the checkpoint kinase Cds1 when DNA replication encounters a stressful condition. PMID: 18440981
nap1 cds1 mutant cells lose viability and cells show positive histone phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. PMID: 18474252
Mrc1 that prevent activation of Cds1 have no effect on its ability to support rid mutant viability, suggesting that Mrc1 has a checkpoint-independent role in maintaining the viability of mutants defective in DNA replication initiation PMID: 18667534
Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its dissociation from the MBF transcription factor. PMID: 18682565
Cds1-dependent stabilization of Rep2 is achieved through the inhibition/phosphorylation of APC/C-Ste9 at the onset of S-phase arrest. PMID: 19596787

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Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily

Database Links

KEGG: spo:SPCC18B5.11c

STRING: 4896.SPCC18B5.11c.1

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cds1 Antibody

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