Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase cds1(cds1)

Code CSB-YP600600SXV
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Source Yeast
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Code CSB-EP600600SXV
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Source E.coli
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Code CSB-EP600600SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600600SXV
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Source Baculovirus
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Code CSB-MP600600SXV
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names cds1
Uniprot No. Q09170
Alternative Names cds1; SPCC18B5.11cSerine/threonine-protein kinase cds1; EC 2.7.11.1; Checkpoint kinase cds1
Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region 1-460
Target Protein Sequence MEEPEEATQA TQEAPLHVSQ NIAKQVVNNE NVFMKLVMTR MLDGKTEVIP LTTDVHNGFW RFGRHKSCEV VLNGPRVSNF HFEIYQGHRN DSDESENVVF LHDHSSNGTF LNFERLAKNS RTILSNGDEI RIGLGVPKDE ISFLCQVPVK HSRDSQKNMI KSENSHYEII RTLGSGTFAV VKLAVEVNSG KWYAIKIINK RKILLTSSEK RATEMFQREI DILKSLHHPG VVQCHEIFEN DDELFIVMEY VEGGDLMDFL IANGSIDEQD CKPLLKQLLE TLLHLHKQGV THRDIKPENI LITNDFHLKI SDFGLAKVIH GTGTFLETFC GTMGYLAPEV LKSKNVNLDG GYDDKVDIWS LGCVLYVMLT ASIPFASSSQ AKCIELISKG AYPIEPLLEN EISEEGIDLI NRMLEINPEK RISESEALQH PWFYTVSTHE HRTPPSSSEH EATEQLNSSS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Has a role in the DNA replication-monitoring S/G2 checkpoint system. It is responsible for blocking mitosis in the S phase. It monitors DNA synthesis by interacting with DNA polymerase alpha and sends a signal to block the onset of mitosis while DNA synthesis is in progress. Phosphorylates rad60 and dna2.
Gene References into Functions
  1. The prolongation in mutants deficient in meiotic DNA replication, was caused by the Cds1-dependent replication checkpoint, which represses expression of the mei4(+) gene encoding a meiosis-specific transcription factor. PMID: 25492408
  2. Cki3 acts downstream of the checkpoint kinase Cds1/Chk2 and calcineurin, and the latter physically interacts with Cki3. PMID: 25691662
  3. Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6. PMID: 19755492
  4. the existence in fission yeast of a short variant (Sv) of Cds1 that is produced through an alternative splicing mechanism leading to a frame shift and premature termination PMID: 15467464
  5. Replication checkpoint kinase Cds1 regulates Mus81 (a subunit of the Mus81-Eme1 structure-specific endonuclease) by phosphorylation, to preserve genome integrity during replication stress. PMID: 15805465
  6. Rad3-Cds1 meiotic checkpoint pathway may target transcription of these factors. PMID: 16286472
  7. DNA-damage checkpoint pathways mediated by Cds1 and Rad3. PMID: 18154680
  8. These data show that by releasing Flp1p into the nucleus Cds1p checkpoint kinase modulates its own full activation during replication stress. PMID: 18385517
  9. Clr6-HDAC (histone deacetylase) regulates the checkpoint kinase Cds1 when DNA replication encounters a stressful condition. PMID: 18440981
  10. nap1 cds1 mutant cells lose viability and cells show positive histone phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. PMID: 18474252
  11. Mrc1 that prevent activation of Cds1 have no effect on its ability to support rid mutant viability, suggesting that Mrc1 has a checkpoint-independent role in maintaining the viability of mutants defective in DNA replication initiation PMID: 18667534
  12. Phosphorylation of Nrm1 by the Cds1 (Chk2) checkpoint protein kinase, which is activated in response to DNA replication stress, promotes its dissociation from the MBF transcription factor. PMID: 18682565
  13. Cds1-dependent stabilization of Rep2 is achieved through the inhibition/phosphorylation of APC/C-Ste9 at the onset of S-phase arrest. PMID: 19596787

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Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily
Database Links

KEGG: spo:SPCC18B5.11c

STRING: 4896.SPCC18B5.11c.1

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