TAH11 Antibody

Code CSB-PA342459XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TAH11 Polyclonal antibody
Uniprot No.
Target Names
TAH11
Alternative Names
TAH11 antibody; CDT1 antibody; SID2 antibody; YJR046W antibody; J1641Cell division cycle protein CDT1 antibody; SIC1 indispensable protein 2 antibody; Topoisomerase-A hypersensitive protein 11 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) TAH11 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.
Gene References into Functions
  1. Cdt1 acts as a brace, holding MCM open for DNA entry and bound to ATP until ORC-Cdc6 triggers ATP hydrolysis by MCM, promoting both Cdt1 ejection and MCM ring closure in Saccharomyces cerevisiae PMID: 28643783
  2. theses data support a model in which origin-bound ORC and Cdc6 recruit two Cdt1 molecules to initiate double-hexamer formation prior to helicase loading and demonstrate that Cdt1 influences the replication competence of loaded Mcm2-7 helicases. PMID: 22045335
  3. Consistent with the inactivation of one Cdt1-binding site preventing helicase loading, CDK phosphorylation of ORC causes a twofold reduction of initial Cdt1/Mcm2-7 recruitment but results in nearly complete inhibition of Mcm2-7 loading. PMID: 21289063
Subcellular Location
Cytoplasm. Nucleus. Note=Undergoes cell cycle-dependent changes in its nuclear localization. Exits the nucleus and remains in the cytoplasm during S phase through early mitosis, and re-accumulates in the nucleus around the end of mitosis.
Protein Families
Cdt1 family
Database Links

KEGG: sce:YJR046W

STRING: 4932.YJR046W

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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