CERK Antibody

Code CSB-PA276403XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CERK Polyclonal antibody
Uniprot No.
Target Names
CERK
Alternative Names
CERK antibody; ACD5 antibody; At5g51290Ceramide kinase antibody; AtCERK antibody; EC 2.7.1.138 antibody; Protein ACCELERATED CELL DEATH 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CERK protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Possesses high activity on ceramide analogs (C6, C8 synthetic ceramides) and lower activity on C6 and C8 dihydroceramides. Has weak activity on natural ceramides (a mixture of ceramides from bovine brain) and the synthetic substrate C2 ceramide. Has very poor activity on diacylglycerol and sphingosine. Ceramide is a critical sphingolipid metabolite that induces programmed cell death (PCD) in plants and ceramide-1-phosphate has a PCD suppressive effect. Thus, ceramide phosphorylation plays a role in the modulation of PCD and CERK activity is crucial for the maintenance of cell viability.
Gene References into Functions
  1. The data involve for the first time ceramide phosphates (Cer-Ps) and ACD5 in low temperature response and further underline the complexity of sphingolipid signalling operating during cold stress. PMID: 26013074
  2. ACD5 is associated with cermide accumulation and Arabidopsis antifungal response. [ACD5] PMID: 25149397
Database Links

KEGG: ath:AT5G51290

STRING: 3702.AT5G51290.1

UniGene: At.21774

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