cfa Antibody

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Code	CSB-PA364333XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) cfa Polyclonal antibody

Uniprot No.

P0A9H7

Target Names

cfa

Alternative Names

cfa antibody; cdfA antibody; b1661 antibody; JW1653Cyclopropane-fatty-acyl-phospholipid synthase antibody; CFA synthase antibody; Cyclopropane fatty acid synthase antibody; EC 2.1.1.79 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) cfa protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge.

Gene References into Functions

both chemical steps of this enzymatic cyclopropanation, the methyl addition onto the double bond and the deprotonation step, are rate determining, a common situation in efficient enzymes. PMID: 20538038
Experiments are described that distinguish between two mechanistic scenarios for the cyclopropanation of unactivated olefins by cyclopropane fatty acid synthase. PMID: 15491158
report mutagenetic and chemical rescue experiments that confirm an important role for bicarbonate in E. coli CFAS catalysis; propose that residue E239, one of the bicarbonate ligands, could form a catalytic dyad with this ion PMID: 16216082

Subcellular Location

Cytoplasm.

Protein Families

CFA/CMAS family

Database Links

KEGG: ecj:JW1653

STRING: 316385.ECDH10B_1795

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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cfa Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background