cfa Antibody

Code CSB-PA364333XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cfa Polyclonal antibody
Uniprot No.
Target Names
cfa
Alternative Names
cfa antibody; cdfA antibody; b1661 antibody; JW1653Cyclopropane-fatty-acyl-phospholipid synthase antibody; CFA synthase antibody; Cyclopropane fatty acid synthase antibody; EC 2.1.1.79 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cfa protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge.
Gene References into Functions
  1. both chemical steps of this enzymatic cyclopropanation, the methyl addition onto the double bond and the deprotonation step, are rate determining, a common situation in efficient enzymes. PMID: 20538038
  2. Experiments are described that distinguish between two mechanistic scenarios for the cyclopropanation of unactivated olefins by cyclopropane fatty acid synthase. PMID: 15491158
  3. report mutagenetic and chemical rescue experiments that confirm an important role for bicarbonate in E. coli CFAS catalysis; propose that residue E239, one of the bicarbonate ligands, could form a catalytic dyad with this ion PMID: 16216082
Subcellular Location
Cytoplasm.
Protein Families
CFA/CMAS family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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