Product Details

Full Product Name

Rabbit anti-Rattus norvegicus (Rat) Cth Polyclonal antibody

Uniprot No.

P18757

Target Names

Cth

Alternative Names

Cth antibody; Cystathionine gamma-lyase antibody; EC 4.4.1.1 antibody; Cysteine-protein sulfhydrase antibody; Gamma-cystathionase antibody; Probasin-related antigen antibody; PRB-RA antibody

Raised in

Rabbit

Species Reactivity

Rattus norvegicus (Rat)

Immunogen

Recombinant Rattus norvegicus (Rat) Cth protein

Immunogen Species

Rattus norvegicus (Rat)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.

Gene References into Functions

indings show that brief and repeated ischemic postconditioning increased the expression of CSE after ischemia/reperfusion in diabetes mellitus PMID: 29880385
Delayed gastric emptying in diabetic rats is due to down-regulation of the hydrogen sulfide biosynthesis enzyme, cystathionine gamma lyase. PMID: 27324218
These findings indicated that nitric oxide mediated the stimulatory effect of gastric acid on hydrogen sulfide release and protein expression of cystathione gamma lyase. PMID: 26319795
Up Regulation of cystathione gamma lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol-Caffeine Induced Left Ventricular Hypertrophy PMID: 26963622
Overexpression of cystathionine gamma-lyase protects mesenchymal stem cells from hypoxia and serum deprivationinduced apoptosis in vitro. PMID: 25901909
demonstrates for the first time that both hyperglycemia and hyperketonemia mediate a reduction in CSE expression and activity, which can contribute to the impaired H2S signaling associated with diabetes PMID: 24610811
Treatment of mesangial cells with platelet-derived growth factor-BB resulted in a marked upregulation of cystathionine-gamma-lyase expression and activity accompanied by increased formation of reactive oxygen species. PMID: 22428706
L-Arginine reverses the down-regulation of CSE/H2S system in high pulmonary blood flow-induced pulmonary hypertension. PMID: 21158086
These findings suggest calcium signaling-dependent cystathionine gamma-lyase expression may be mediated via protein phosphorylation of Sp1 and Elk1 in pancreatic beta-cells. PMID: 22133746
HgCl(2), CH(3)Hg-S-Cys and Cys-S-Hg-S-Cys are potent irreversible inhibitors of rat cystathionine gamma-lyase. PMID: 22093698
Overexpression of rat cystathionine gamma-lyase (CSE) stimulates human vascular smooth muscle cell apoptosis due to an increased endogenous production of H2S. PMID: 16507767
The serum levels of H(2)S and CSE activity were significantly increased in hepatic ischemia reperfusion injury. PMID: 21055229
lack of gamma-cystathionase activity in over 50% of old lenses is due to decreased gene expression and proteolytic degradation of the oxidized enzyme; this results in a high risk for the development of senile cataract PMID: 15683713
Increased hepatic gamma-cystathionase activity in the fetal-to-neonatal transition is due to up-regulation of its gene expression mediated by stress hormones together with the physiological oxidative stress that occurs at birth. PMID: 18184479

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Subcellular Location

Cytoplasm.

Protein Families

Trans-sulfuration enzymes family

Database Links

KEGG: rno:24962

STRING: 10116.ENSRNOP00000058914

UniGene: Rn.3881

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Cth Antibody

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