CGS1 Antibody

Code CSB-PA347576XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CGS1 Polyclonal antibody
Uniprot No.
Target Names
CGS1
Alternative Names
CGS1 antibody; CYS1 antibody; MTO1 antibody; At3g01120 antibody; T4P13.19Cystathionine gamma-synthase 1 antibody; chloroplastic antibody; AtCGS1 antibody; EC 2.5.1.48 antibody; METHIONINE OVERACCUMULATION 1 antibody; O-succinylhomoserine antibody; thiol)-lyase antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CGS1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction. Substrate preference for cystathionine synthesis is O-phospho-L-homoserine (OPH) > O(4)-succinyl-L-homoserine (OSH) >> O-acetyl-L-homoserine (OAH). Is able, at extremely low rate, to catalyze a gamma-elimination of OPH in the absence of cysteine to produce inorganic phosphate (Pi), 2-oxobutanoate and ammonia.
Gene References into Functions
  1. Seed-specific repression of AtCGS in RNA interference seeds triggered the induction of genes operating in the SMM cycle of rosette leaves. PMID: 28536103
  2. Cystathione-gamma-synthase stimulates metabolic and transcriptomic responses associated with desiccation stress in seeds. PMID: 25232013
  3. Report shows that the poly(A) tail of the full-length CGS1 mRNA showed an apparent increase from 50-80 nucleotides (nt)to 140-150 nt after the induction of CGS1 mRNA degradation. PMID: 24463527
  4. Data indicate that stacked ribosomes play in the control of cystathionine gamma-synthase CGS1 mRNA degradation. PMID: 24652291
  5. Light and sucrose up-regulate the expression level of cystathionine gamma-synthase. PMID: 23933995
  6. The transcript of the N-terminus region deleted form of CGS is insensitive to methionine application and its expression may be important for maintaining methionine metabolism even in the presence of a high level of methionine. PMID: 16507086
  7. The results of this study suggest that a metabolic effector, to date unidentified, can modulate CGS activity in vivo through an interaction with the N-terminal domain of the enzyme and that removal of this domain can suppress this regulation. PMID: 17720756
  8. These results show that S-adenosyl-L-methionine induces the stacking of ribosomes on CGS1 mRNA and that multiple mRNA degradation sites probably correspond to each stacked ribosome. PMID: 18184691
  9. CGS1 mRNA degradation involves a plant-specific mechanism. PMID: 18285355

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Subcellular Location
Plastid, chloroplast.
Protein Families
Trans-sulfuration enzymes family
Database Links

KEGG: ath:AT3G01120

STRING: 3702.AT3G01120.1

UniGene: At.20198

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