chk-1 Antibody

Code CSB-PA865057XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans chk-1 Polyclonal antibody
Uniprot No.
Target Names
chk-1
Alternative Names
chk-1 antibody; Y39H10A.7 antibody; Serine/threonine-protein kinase chk-1 antibody; EC 2.7.11.1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans chk-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Required for checkpoint mediated cell cycle arrest in response to DNA damage in germline cells. Delays cell-cycle reentry of the Z2 and Z3 primordial germ cells in response to transcription-induced DNA damage as they emerge from cell cycle arrest in L1 larvae. Essential for embryogenesis.
Gene References into Functions
  1. Chk-1 had an essential role in C. elegans embryo and germline development. More specifically, disruption of chk-1 expression resulted in embryo lethality, which was attributed to a defect in an intrinsic S-M checkpoint. PMID: 15326393
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, perinuclear region.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
Tissue Specificity
Expressed in the germline.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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