CHL1 Antibody

Code CSB-PA324430XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CHL1 Polyclonal antibody
Uniprot No.
Target Names
CHL1
Alternative Names
CHL1 antibody; CTF1 antibody; YPL008W antibody; YP8132.05ATP-dependent DNA helicase CHL1 antibody; EC 3.6.4.12 antibody; Chromosome loss protein 1 antibody; Chromosome transmission fidelity protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CHL1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.
Gene References into Functions
  1. The Chl1 helicase facilitates replication fork progression under conditions of nucleotide depletion, partly independently of Ctf4 interaction. PMID: 27397686
  2. Chl1 DNA helicase regulates Scc2 deposition specifically during DNA-replication in Saccharomyces cerevisiae. PMID: 24086532
  3. study shows that Chl1p is required for normal growth and cell viability in the presence of the replication block caused by HU. PMID: 21943249
  4. Chl1p may have a role in regulating chromatin structure. PMID: 16182251
  5. Chl1p is required for genome integrity when cells suffer endogenously or exogenously induced DNA damage. PMID: 17062629

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Subcellular Location
Nucleus.
Protein Families
DEAD box helicase family, DEAH subfamily, DDX11/CHL1 sub-subfamily
Database Links

KEGG: sce:YPL008W

STRING: 4932.YPL008W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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