Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHLH Polyclonal antibody

Uniprot No.

Q9FNB0

Target Names

CHLH

Alternative Names

CHLH antibody; ABAR antibody; CCH antibody; GUN5 antibody; RTL1 antibody; At5g13630 antibody; MSH12.9Magnesium-chelatase subunit ChlH antibody; chloroplastic antibody; Mg-chelatase subunit H antibody; EC 6.6.1.1 antibody; ABA-binding protein antibody; Mg-protoporphyrin IX chelatase subunit ChlH antibody; Protein CONDITIONAL CHLORINA antibody; Protein GENOMES UNCOUPLED 5 antibody; Protein RAPID TRANSPIRATION IN DETACHED LEAVES 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CHLH protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Multifunctional protein involved in chlorophyll synthesis, plastid-to-nucleus retrograde signaling and abscisic acid (ABA) perception. In chlorophyll synthesis, catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. In addition to its function in the Mg-chelatase enzyme, is required for the plastid-to-nucleus retrograde signaling. The plastid-to-nucleus signal plays an important role in the coordinated expression of both nuclear- and chloroplast-localized genes that encode photosynthesis-related proteins. Has a role in mediating ABA signaling in stomatal guard cells and during seed germination. Binds ABA and is a positive regulator of ABA signaling.

Gene References into Functions

Gun5 role in chloroplast development and tetrapyrrole biosynthesis PMID: 27001427
findings provide clear evidence that the Mg-chelatase-catalyzed Mg-ProtoIX production is distinct from ABA signaling, giving information to understand the mechanism by which the two cellular processes differs at the molecular level PMID: 23011401
A missense mutation of CHLH (L690 to F) is responsible for phenotype in which stomatal movements are insensitive to abscisic acid. PMID: 21562844
ABAR spans the chloroplast envelope and that the cytosolic C terminus of ABAR interacts with a group of WRKY transcription factors that function as negative regulators of ABA signaling in seed germination and postgermination growth. PMID: 20543028
Timing of chlorophyll-AB expression protein TOC1 is induced by abscisic acid (ABA)signaling; this induction is gated by the clock and determines the timing of TOC1 binding to the ABA-related gene promoter. PMID: 19816401
Arabidopsis ABAR/CHLH specifically binds ABA (abscisic acid), and mediates ABA signalling as a positive regulator in seed germination, post-germination growth and stomatal movement, showing that ABAR/CHLH is an ABA receptor [ABAR] PMID: 17051210
ABAR/CHLH is an ABA receptor and reveal that the C-terminal half of ABAR/CHLH plays a central role in ABA signaling. PMID: 19535472

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Subcellular Location

Plastid, chloroplast stroma. Plastid, chloroplast membrane; Peripheral membrane protein; Stromal side. Plastid, chloroplast membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

Mg-chelatase subunit H family

Tissue Specificity

Widely expressed.

Database Links

KEGG: ath:AT5G13630

STRING: 3702.AT5G13630.1

UniGene: At.23621

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CHLH Antibody

Product Details

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Target Background