Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHR25 Polyclonal antibody

Uniprot No.

Q0PCS3

Target Names

CHR25

Alternative Names

CHR25 antibody; RAD54 antibody; At3g19210 antibody; MVI11.13 antibody; Protein CHROMATIN REMODELING 25 antibody; AtCHR25 antibody; EC 3.6.4.- antibody; DNA repair and recombination protein RAD54 antibody; AtRAD54 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CHR25 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments. Addition of RAD54 overcomes inhibition of DNA strand exchange by RAD51 bound to substrate dsDNA. Species preference in the RAD51 dissociation and DNA strand exchange assays underlines the importance of specific RAD54-RAD51 interactions. RAD51 is unable to release dsDNA upon ATP hydrolysis, leaving it stuck on the heteroduplex DNA product after DNA strand exchange. Involved in DNA repair and mitotic recombination. Functions in the homologous recombinational DNA repair (RAD52) pathway. Required for synthesis-dependent strand annealing (SDSA) during double-strand break repair.; Facilitates geminiviral replication (e.g. geminivirus mungbean yellow mosaic virus (MYMV) and tomato leaf curl virus (ToLCV)).

Gene References into Functions

The appearance of RAD54 foci was dependent on the ATAXIA-TELANGIECTASIA MUTATED-SUPPRESSOR OF GAMMA RESPONSE 1 pathway, and RAD54 foci were co-localized with gammaH2AX signals. Laser irradiation of a subnuclear area demonstrated that in living cells RAD54 was specifically accumulated at the damaged site. In addition, the formation of RAD54 foci showed specificity for cell type and region. We conclude that RAD54 foci co... PMID: 28155243
Study identified a host factor, RAD54, in a screen for replication initiator protein (Rep)-interacting partners and observed its role in DNA replication of the geminivirus mungbean yellow mosaic India virus (MYMIV). PMID: 22171001
findings point to an important role for Rad54 in homologous recombination repair in plants [Rad54] PMID: 17227544
The trans-species compatibility of Rad54 functions, is reported. PMID: 18430956

Subcellular Location

Nucleus.

Protein Families

SNF2/RAD54 helicase family

Tissue Specificity

Expressed ubiquitously, with the highest levels of expression in flower buds. Present in flower buds (at protein level).

Database Links

KEGG: ath:AT3G19210

STRING: 3702.AT3G19210.1

UniGene: At.53386

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CHR25 Antibody

Product Details

Customer Reviews and Q&A

Target Background