CIK1 Antibody

Code CSB-PA935136XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CIK1 Polyclonal antibody
Uniprot No.
Target Names
CIK1
Alternative Names
CIK1 antibody; YMR198W antibody; YM9646.11Spindle pole body-associated protein CIK1 antibody; Chromosome instability and karyogamy protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CIK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
A developmentally regulated protein important for microtubule functions. Targets KAR3 to the cytoplasmic microtubules during mating. Essential for KAR3 function during prophase of meiosis I. Required for interhomolog recombination, synapsis of homologous chromosomes and establishment of a meiosis I spindle.
Gene References into Functions
  1. Cryoelectron microscopy generated 3-D structure of Kar3Cik1 complexed to microtubules in various nucleotide states. PMID: 24099757
  2. we found that Cik1 mediates the association of Kar3 with kinetochores and this association only occurs before chromosome bipolar attachment. PMID: 22319456
  3. a model in which Kar3Cik1 interacts with the MT lattice through an alternating cycle of Cik1 MT collision followed by Kar3 MT binding with head-head communication between Kar3 and Cik1 modulated by the Kar3 nucleotide state and intramolecular strain PMID: 21680740
  4. Cik1 targets the minus-end kinesin depolymerase kar3 to microtubule plus ends. PMID: 16085496
  5. structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo PMID: 16107877

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
Database Links

KEGG: sce:YMR198W

STRING: 4932.YMR198W

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