CIPK11 Antibody

Code CSB-PA521261XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CIPK11 Polyclonal antibody
Uniprot No.
Target Names
CIPK11
Alternative Names
CIPK11 antibody; PKS5 antibody; SIP4 antibody; SnRK3.22 antibody; At2g30360 antibody; T9D9.17CBL-interacting serine/threonine-protein kinase 11 antibody; EC 2.7.11.1 antibody; SNF1-related kinase 3.22 antibody; SOS2-like protein kinase PKS5 antibody; SOS3-interacting protein 4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CIPK11 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Acts as a negative regulator of the plasma membrane proton pump AHA2 by preventing its interaction with 14-3-3 protein.
Gene References into Functions
  1. PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the abscissic acid regulation of seed germination and gene expression in Arabidopsis. PMID: 25858916
  2. PKS5 involves in external high pH signal mediated microfilament depolymerization, and that may be independent of calcium signal. PMID: 21777855
  3. results suggest that PKS5 functions at the upstream of NPR1 and might mediate expression of WRKY38 and WRKY62 possibly by interacting with and phosphorylating NPR1. PMID: 20621018
  4. J3 regulates plasma membrane H+-ATPase activity through interaction with the PKS5 kinase. PMID: 20418496
  5. These results suggest that PKS5 is part of a calcium-signaling pathway mediating PM H+ -ATPase regulation. PMID: 17483306

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Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Expressed in roots and stems, but barely detectable in flowers and siliques.
Database Links

KEGG: ath:AT2G30360

STRING: 3702.AT2G30360.1

UniGene: At.12244

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