CIPK23 Antibody

Datasheet

Code	CSB-PA842405XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CIPK23 Polyclonal antibody

Uniprot No.

Q93VD3

Target Names

CIPK23

Alternative Names

CIPK23 antibody; LKS1 antibody; PKS17 antibody; SnRK3.23 antibody; At1g30270 antibody; F12P21.6CBL-interacting serine/threonine-protein kinase 23 antibody; EC 2.7.11.1 antibody; Protein LOW-K(+)-SENSITIVE 1 antibody; SNF1-related kinase 3.23 antibody; SOS2-like protein kinase PKS17 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CIPK23 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein leads to activation of the kinase in a calcium-dependent manner. Downstream of CBL1, CBL2, CBL3 and CBL9, regulates by phosphorylation the K(+) conductance and uptake of AKT1 in low K(+) condition, in response to calcium signaling and during the stomatal opening regulation by monitoring the turgor pressure in guard cells. In response to low nitrate concentration, phosphorylates NRT1.1, switching it from a low-affinity nitrate transporter to a high-affinity transporter. Confers tolerance to low potassium conditions. Involved in drought sensitivity and leaf transpiration.

Gene References into Functions

CIPK23 occupies a key position in controlling ion balance and ion homeostasis in the plant cell. PMID: 28188265
Studies highlight that calcium-dependent CBL-CIPK pathway is involved in response to iron deficiency in Arabidopsis. [CIPK23] PMID: 26993237
CIPK23 rendered Arabidopsis mutants hypersensitive to iron deficiency. PMID: 26993237
protein kinase CIPK23, encoded by the LKS1 gene, regulates K+ uptake under low-K+ conditions PMID: 16814720

Subcellular Location

Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when associated with AKT1, CBL1 and CBL9.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily

Tissue Specificity

In seedlings, mostly in vascular bundles, and in roots, especially in cortex and endodermis cells. In adult plants, mostly expressed in flowers, and, to a lower extent, in roots, leaves, stems and siliques, particularly in vascular tissues. Also detected

Database Links

KEGG: ath:AT1G30270

STRING: 3702.AT1G30270.1

UniGene: At.17201

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CIPK23 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background