CDK5RAP1 Antibody

Code CSB-PA822305XA01HU
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CDK5RAP1 Polyclonal antibody
Uniprot No.
Target Names
CDK5RAP1
Alternative Names
CDK5RAP1 antibody; C20orf34 antibody; CGI-05 antibody; HSPC167CDK5 regulatory subunit-associated protein 1 antibody; CDK5 activator-binding protein C42 antibody
Raised in
Rabbit
Species Reactivity
Homo sapiens (Human)
Immunogen
Recombinant Homo sapiens (Human) CDK5RAP1 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Methylthiotransferase that catalyzes the conversion of N6-(dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp). Essential for efficient and highly accurate protein translation by the ribosome. Specifically inhibits CDK5 activation by CDK5R1. Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease.
Gene References into Functions
  1. The mitochondrial localization of ms2i6A modification was diminished in HeLa cells when transfected with siRNA against Cdk5 regulatory subunit-associated protein 1 (Cdk5rap1), suggesting that the modification occurs in tRNAs in mitochondria. PMID: 28981754
  2. Which resulted in respiratory defects in Cdk5rap1 knockout (KO) mice. PMID: 25738458
  3. data indicated that CDK5RAP1 deficiency induced cell cycle arrest and apoptosis in human breast cancer PMID: 25607831
  4. CDK5RAP1 may be a risk factor of vitiligo in the Korean population PMID: 22534366
  5. CDK5RAP1 is a radical SAM enzyme, which postsynthetically converts the RNA modification N6-isopentenyladenosine into 2-methylthio-N6-isopentenyladenosine. PMID: 22422838

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Subcellular Location
Mitochondrion.
Protein Families
Methylthiotransferase family, MiaB subfamily
Tissue Specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in neurons of central nervous tissue.; [Isoform 5]: Mainly expressed in brain, placenta and testis.; [Isoform 6]: High expression in placenta and lung.
Database Links

HGNC: 15880

OMIM: 608200

KEGG: hsa:51654

STRING: 9606.ENSP00000217372

UniGene: Hs.435952

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