ema Antibody

Datasheet

Code	CSB-PA714256XA01DLU
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) ema Polyclonal antibody

Uniprot No.

Q9VEV4

Target Names

ema

Alternative Names

ema antibody; CG12753Protein CLEC16A homolog antibody; Endosomal maturation defective protein antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) ema protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Required for mitophagy, autophagy and endosome maturation, possibly by acting in multiple membrane trafficking pathways. Required for endosome trafficking and maturation. Functions with the class C Vps-HOPS complex member Vps16a to promote endosomal maturation into degradative late endosomes and lysosomes. In response to starvation, functions at an early stage of autophagy to promote autophagosome growth and efficient autophagy. Essential for the recruitment of lva-positive Golgi elements to autophagosomes. Likely to function by promoting membrane traffic from the Golgi complex to the developing autophagosomes. Also regulates synaptic growth at the neuromuscular junctions (NMJ) by down-regulating BMP signaling.

Gene References into Functions

This study not only identifies a role for Ema in autophagy, but also supports the hypothesis that the Golgi complex may be a potential membrane source for the biogenesis and development of autophagic structures. PMID: 22713284
In fat body cells, Ema localizes to the Golgi complex and is recruited to the membrane of autophagosomes in response to starvation. PMID: 22493244

Subcellular Location

Cytoplasmic vesicle, autophagosome membrane; Single-pass membrane protein. Late endosome membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein.

Protein Families

CLEC16A/gop-1 family

Database Links

KEGG: dme:Dmel_CG12753

STRING: 7227.FBpp0082766

UniGene: Dm.10871

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