CLC2 Antibody

Code CSB-PA522347XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLC2 Polyclonal antibody
Uniprot No.
Target Names
CLC2
Alternative Names
CLC2 antibody; At2g40060 antibody; T28M21.22Clathrin light chain 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CLC2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Gene References into Functions
  1. Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing blue light-triggered establishment of asymmetric auxin distribution. PMID: 27770560
  2. Majority of clathrin light chain with green fluorescent protein (CLC-GFP) is localized to the trans-Golgi network (TGN), and a minor population is associated with multivesicular endosomes and the Golgi trans-cisternae. PMID: 21910772
  3. Data indicate that MTV1 (AT3G16270) and AGD5 (AT5G54310) Physically Interact with clathrin. PMID: 23771894
  4. At2g40060 regulates clathrin-mediated trafficking, auxin signaling, and development in Arabidopsis. PMID: 23424247
Subcellular Location
Cell membrane. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, phragmoplast.
Protein Families
Clathrin light chain family
Tissue Specificity
Expressed in root epidermal cells and root hairs (at the protein level).
Database Links

KEGG: ath:AT2G40060

STRING: 3702.AT2G40060.1

UniGene: At.22455

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