clcA Antibody

1. Thermodynamic analysis of ion and proton binding shows how the structure of the CLC transporter effects the pH-independent, 2 Cl- to 1 proton binding stoichiometry. The key features are a helix cage, which creates a compact region with a very strong electropositive potential that stabilizes two anions and a single Glutamate that competes with two Cl ions. PMID: 28877486
2. Crystallographic study on the ion-binding properties of the ClC selectivity filter in the close homolog from Escherichia coli was presented. PMID: 16341087
3. The unitary Cl(-) transport rate, measured in liposomes by both initial-velocity method and a novel Poisson dilution approach, is approximately 4,000 s(-1) for wild-type protein, and the uncoupled mutants transport Cl(-) at similar rates PMID: 17389248
4. E203 substitution with Cys and crystal structures of mutants showed that respective Glu is the internal proton-transfer residue that delivers protons from intracellular solution to the protein interior PMID: 19139174
5. The authors propose that CLC-ec1 operates through an affinity-switch mechanism and that the bases of substrate specificity are conserved in the CLC channels and transporters. PMID: 19898476

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KEGG: ecj:JW5012

STRING: 316385.ECDH10B_0135