CLE45 Antibody

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Code	CSB-PA750357XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLE45 Polyclonal antibody

Uniprot No.

Q6IWA9

Target Names

CLE45

Alternative Names

CLE45 antibody; At1g69588 antibody; F24J1CLAVATA3/ESR antibody; CLE)-related protein 45 [Cleaved into: CLE45p] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CLE45 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Extracellular signal peptide that regulates cell fate. Represses root apical meristem maintenance. Represses protophloem differentiation in a BAM3-dependent manner. BRX, BAM3, and CLE45 act together to regulate the transition of protophloem cells from proliferation to differentiation, thus impinging on postembryonic growth capacity of the root meristem; this signaling pathway requires CRN and CLV2 and involves MAKR5 for its transduction/amplification. Triggers the accumulation of MAKR5 in developing sieve elements in a BAM3-dependent manner. Prevents, in a dose-dependent manner, auxin response in the root meristem thus leading in the repression of protophloem differentiation and periclinal sieve element precursor cell division. Promotes pollen tube growth prolongation in a SKM1 and SKM2-dependent manner, especially under relatively high temperature (at 30 degrees Celsius), thus conferring tolerance against high temperature probably through the maintenance of mitochondrial activity. Alleviates mitochondrial decay pollen tube in vitro culture.

Gene References into Functions

study uncovers BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface; CRN stabilizes BAM3 expression and thus is required for BAM3-mediated CLE45 signaling PMID: 28607033
results strongly suggest that the pollen-pistil interaction via the CLE45-SKM1/SKM2 signaling pathway sustains pollen performance under higher temperatures, leading to successful seed production PMID: 23910659

Subcellular Location

[CLE45p]: Secreted, extracellular space.

Protein Families

CLV3/ESR signal peptide family

Tissue Specificity

Expressed at low levels in flowers, especially in pistils. Present in vascular tissues. In roots, confined to protophloem and sieve element precursor cells.

Database Links

KEGG: ath:AT1G69588

STRING: 3702.AT1G69588.1

UniGene: At.69448

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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CLE45 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background