Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLF Polyclonal antibody

Uniprot No.

P93831

Target Names

CLF

Alternative Names

CLF antibody; ICU1 antibody; PIF1 antibody; PIF2 antibody; SDG1 antibody; SET1 antibody; At2g23380 antibody; F26B6.3Histone-lysine N-methyltransferase CLF antibody; EC 2.1.1.- antibody; Polycomb group protein CURLY LEAF antibody; Protein INCURVATA 1 antibody; Protein SET DOMAIN GROUP 1 antibody; Protein photoperiod insensitive flowering antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CLF protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to regulate floral development by repressing the AGAMOUS homeotic gene in leaves, inflorescence stems and flowers. Together with ATX1, modulates AG nucleosome methylation statement. Regulates the antero-posterior organization of the endosperm, as well as the division and elongation rates of leaf cells. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development.

Gene References into Functions

CLF represses a large number of genes in embryos at the mature-green stage.CLF regulates gene sets coordinating seed size and lipid biosynthesis. PMID: 26945048
We show that CURLY LEAF (CLF), a major catalytic subunit of PRC2, coordinates with different members of PRC1 in suppression of distinct plant developmental programs. PMID: 26760036
CLF may also regulate homologous recombination via other mechanisms. PMID: 24393343
CLF is required for the temporally regulated termination of floral stem cell maintenance. PMID: 22028461
Antagonistic roles of SEPALLATA3, FT and FLC genes as targets of the polycomb group gene CURLY LEAF PMID: 22363474
Loss of CLF function increases meristematic activity in Arabidopsis. PMID: 21441433
BLI colocalizes with CLF in the nucleus, shows an overlapping expression pattern with CLF throughout plant development that is strongest in dividing cells, and represses a subset of Pc-G target genes PMID: 20647345
A mutation in CLF suppresses FLC-mediated delayed flowering via reduced FLC expression. PMID: 19755537
the spread of H3K27me3 contributes to the mitotic heritability of Polycomb-group (Pc-G) silencing, and that the loss of silencing caused by transposon insertions at plant Pc-G targets reflects impaired spreading. PMID: 16957776
The Arabidopsis homologs of trithorax (ATX1) and enhancer of zeste (CLF) interact to control the expression of the flower homeotic gene AGAMOUS. PMID: 17881378
PRC2-like complexes containing CLF, EMF2 and FIE, directly interact with and deposit into FT, FLC and FLC relatives repressive trimethyl H3K27 leading to the suppression of active H3K4me3 in these loci, repressing expression of these flowering genes. PMID: 18852898

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Subcellular Location

Nucleus.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, EZ subfamily

Tissue Specificity

Strongly expressed throughout the apical meristem, leaf primordia, and leaves of 7-8 day-old seedling. Weakly expressed in the vasculature of hypocotyl. Strongly expressed throughout the young stages 1 and 2 floral meristems that arose on the flanks of th

Database Links

KEGG: ath:AT2G23380

STRING: 3702.AT2G23380.1

UniGene: At.22

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CLF Antibody

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Target Background