CLO Antibody

Datasheet

Code	CSB-PA155821XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLO Polyclonal antibody

Uniprot No.

Q9LNC5

Target Names

CLO

Alternative Names

CLO antibody; GFA1 antibody; MEE5 antibody; VAJ-1 antibody; At1g06220 antibody; F9P14.8110 kDa U5 small nuclear ribonucleoprotein component CLO antibody; Protein CLOTHO antibody; Protein GAMETOPHYTE FACTOR 1 antibody; Protein MATERNAL EFFECT EMBRYO ARREST 5 antibody; Protein VAJRA-1 antibody; SNU114 homolog antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CLO protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Splicing factor involved in pre-mRNA splicing and component of the spliceosome. Essential for reproduction. In female gametophyte, is necessary for the egg cell and central cell fate determination and hence reproductive success. Involved in a mechanism that prevents accessory cells from adopting gametic cell fate. Is necessary to restrict LIS expression to interfere with egg-cell specification. Probable component of U5 small nuclear ribonucleoprotein (snRNP) that is required for pre-mRNA splicing. Plays an essential role in female gametogenesis and embryo development. Required for the control of polarized cell growth and cell proliferation during floral organ morphogenesis.

Gene References into Functions

interaction with ROOT INITIATION DEFECTIVE 1 is involved in female gametophyte development PMID: 27683728
CLOTHO/GAMETOPHYTIC FACTOR 1 (CLO/GFA1) is necessary for the restricted expression of egg- and central-cell fate. CLO/GFA1 encodes the Arabidopsis homologue of Snu114, a protein that is considered to be an essential component of the spliceosome. PMID: 18702672
VAJ/GFA1/CLO co-localized with a serine/arginine-rich (SR) protein SC35 in the nuclei and has a novel role in the directional control of floral organ growth in Arabidopsis. PMID: 19168457
GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant. [GFA1] PMID: 19261069

Subcellular Location

Nucleus speckle.

Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family

Tissue Specificity

Expressed in flower buds, open flowers and siliques. Expressed at low levels in rosettes leaves, cauline leaves and stems.

Database Links

KEGG: ath:AT1G06220

STRING: 3702.AT1G06220.1

UniGene: At.15476

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