Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CLP1 Polyclonal antibody

Uniprot No.

Q92989

Target Names

CLP1

Alternative Names

ATP/GTP binding protein antibody; Cleavage and polyadenylation factor I subunit 1 antibody; CLP 1 antibody; clp1 antibody; CLP1 cleavage and polyadenylation factor I subunit homolog antibody; CLP1; cleavage and polyadenylation factor I subunit; homolog (S. cerevisiae) antibody; CLP1; yeast; homolog of antibody; CLP1_HUMAN antibody; hClp1 antibody; Homolog of yeast CFIA subunit Clp1p antibody; Polyadenylation factor Clp1 antibody; Polynucleotide kinase Clp1 antibody; Polyribonucleotide 5' hydroxyl kinase Clp1 antibody; Polyribonucleotide 5''-hydroxyl-kinase Clp1 antibody; Pre mRNA cleavage complex II protein Clp1 antibody; Pre-mRNA cleavage complex II protein Clp1 antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) CLP1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Gene References into Functions

Study reports the identification of a human CLP1 mutation that impairs tRNA splicing in vitro and causes a neurological syndrome involving both the central nervous system (CNS) and peripheral nervous system (PNS). PMID: 24766809
Data link tRNA maturation to neuronal development and neurodegeneration through defective CLP1 function in humans. PMID: 24766810
the kinase hClp1 phosphorylates and licenses synthetic siRNAs to become assembled into RISC for subsequent target RNA cleavage PMID: 17495927
Expression of hClp1 in budding yeast can complement conditional and lethal mutations in the essential 5'-OH RNA kinase module of yeast or plant tRNA ligases. PMID: 18648070
HEAB is a pre-mRNA 3'-end processing factor, which bridges two other pre-mRNA processing factors (CF Im and CPSF) and is involved in cleavage of the pre-mRNA. It can be UV-crosslinked to GTP. PMID: 11060040

Hide All

Involvement in disease

Pontocerebellar hypoplasia 10 (PCH10)

Subcellular Location

Nucleus.

Protein Families

Clp1 family, Clp1 subfamily

Database Links

HGNC: 16999

OMIM: 608757

KEGG: hsa:10978

STRING: 9606.ENSP00000434995

UniGene: Hs.523687

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